Isotopic Determination and Optimum Reaction Conditions of Pantothenic Acid Synthetase
نویسندگان
چکیده
منابع مشابه
Creatine kinase in serum: 1. Determination of optimum reaction conditions.
To establish optimum conditions for creatine kinase (EC 2.7.3.2) activity measurement with the creatine phosphate in equilibrium creatine reaction, we re-examined all kinetics factors relevant to an optimal and standardized enzyme assay at 30 and 25 degrees C. We determined the pH optimum in vaious buffers, considering the effect of the type and concentration of the buffer, as well as the influ...
متن کاملSimulation of Methanol Production Process and Determination of Optimum Conditions
Methanol is one of the most important petrochemical products which is produced in large extents worldwide. Nowadays, it is mostly used as a feed in olefin units for production of synthetic fuels. In this work, methanol production process, under license of Davy Corporation, is simulated by using HYSYS software. The simulations are carried out for steady state condition. The simulation results we...
متن کاملarchitecture and engineering of nanoscale sculptured thin films and determination of their properties
چکیده ندارد.
15 صفحه اولCreatine kinase: re-examination of optimum reaction conditions.
I have re-examined optimum reaction conditions for measurement of creatine kinase (EC 2.7.3.2). The optimum pH is 6.45, and 2,2-bis(hydroxymethyl)-2,2',2''-nitrotriethanol acetate, 200 mmol/liter, is the buffer of choice. Thioglycerol, 20 mmol/liter, is superior for both in-assay reactivation and for storage stability of sera. Fluoride, 25 mmol/liter, a broad inactivator of adenylate kinase (EC...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1973
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.37.1205